Alternative Electron Donors for the Nitrogenase‐like Dark‐Operative Protochlorophyllide Oxidoreductase (DPOR)

Sharing structural and mechanistic properties with the dinitrogen-fixing enzyme nitrogenase, dark-operative protochlorophyllide oxidoreductase (DPOR) participates in biosynthesis of bacteriochlorophyll, subsequently enabling photosynthesis by photosynthetic bacteria. In contrast to light-dependent POR, electron transfer DPOR is coupled hydrolysis adenosine triphosphate for two-electron stereoselective reduction (Pchlide) chlorophyllide (Chlide), a coupling also employed nitrogenases multielectron dinitrogen ammonia. The importance not fully understood these enzymes, electrochemistry valuable technique regulate and/or monitor delivery enzymes. Here, we report three alternative donors that support system, which could enable electroenzymatic studies DPOR. We demonstrate turnover within spectroelectrochemical configuration, permitting formation Chlide be followed utilization